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Objective To optimize the extraction process of total polyphenols in Artemisia capillaris and study the content of it in Henan Province. Methods A single factor optimization combined with response surface optimization was used to establish the optimal method for extracting total polyphenols from Artemisia capillaris, mainly examining the effects of solid-liquid ratio, ethanol volume fraction, extraction time and extraction temperature on the extraction of total polyphenols from Artemisia capillaris. The samples of Artemisia capillaris were taken from 18 cities in Henan Province, and the total polyphenol content in Artemisia capillaris was analyzed based on soil type and distribution area. Results The best values of 4 factors were as follows: Solid-liquid ratio 1:30 (m:V), ethanol concentration 50%, extraction time 72 min, extraction temperature 63 ℃, and the best extraction rate of total polyphenols was 1.85%. With the best values of 4 factors, the total polyphenols in Artemisia capillaris from 18 regions in Henan was determined and analyzed. The highest value appears in Anyang and the lowest value appears in Zhengzhou. According to the types of soil, the tide soil had the highest value of total polyphenols, which was 7.51 mg/g, and the paddy soil had the lowest value of total polyphenols, which was 6.15 mg/g. Conclusion The method optimized by this study is stable, reliable and economy. The model established can predict the extraction rate of total polyphenols well. The content of total polyphenols in Artemisia capillaris from different regions in Henan is discrepant. But the discrepancy is not significant according to the soil types.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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