PDF
Full
Outline
Objective To synthesize a Au-Pt bimetallic nanozymes (Au-PtNFs) with oxidase and peroxidase dual-enzyme activity, and apply them in detection of organophosphorus pesticide. Methods The 3 kinds of Au-PtNFs were prepared through seed-mediated method by adjusting the amount of chloroplatinic acid, and their enzyme activities were determined using 3,3’,5,5’-tetramethylbenzidine (TMB) as the substrate. The nanozyme exhibiting the highest enzyme activity was selected and combined with the catalytic effect of acetylcholinesterase to establish a detection method for organophosphorus pesticide. Results The 3 kinds of prepared nanozymes all had flower-like structures, and the particle size enhanced with the increasement of chloroplatinic acid concentration. Steady-state dynamic experiments showed that all bimetallic nanozymes had dual-enzyme activity, and the best enzyme activity was obtained when the concentration of chloroplatinic acid was 5 mmol/L. Absorption at 620 nm exhibited a good linear correlation with the logarithm of chlorpyrifos concentration in the range of 40-90 nmol/L, and the limit of detection was 1.00 nmol/L. The method demonstrated excellent specificity and anti-interference ability, and achieved great recovery rate in real samples. Color value analysis software was used for the detection of chlorpyrifos based on smartphone. The linear range was 30-100 nmol/L and the limit of detection was 1.06 nmol/L. Conclusion The prepared Au-PtNFs exhibiting dual-enzyme activity are effectively utilized for detection of organophosphorus pesticides, which offers a novel perspective for visual detection of organophosphorus pesticides in fruits and vegetables.
PDF
284
127
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
