PDF
Full
Outline
Objective To optimize the production process of casein peptone with yak milk casein as raw material. Methods The combination of alkaline protease and trypsin was used to hydrolyze yak milk casein. On the basis of single factor experiment, according to the principle of Box-Behnken central combination test design, the response surface analysis method of four factors and three levels was adopted, and the degree of hydrolysis was used as the response value. The response surface and contour line were used to obtain the optimal process conditions for the hydrolysis of yak milk casein by the combined enzyme, and then the yak milk casein peptone product was obtained by acid precipitation, neutralization and finally spray drying. Results The results of single factor showed that the degree of hydrolysis of casein increased with the increase of enzyme concentration. When the amount of enzyme added was higher than 2.5%, the degree of hydrolysis increased slowly. With the increase of temperature and pH, the degree of hydrolysis increased first and then decreased. The degree of hydrolysis of yak milk casein reached the maximum when the substrate concentration was 5%, and the substrate concentration would hinder the effect of the enzyme on the cutting site, resulting in a decrease in protein conversion. The results of response surface showed that the degree of hydrolysis was the best when the amount of combined enzyme was 2.6% (alkaline protease:trypsin=4:1), the hydrolysis time was 160 min, the hydrolysis pH was 7.0, the substrate concentration was 5%, and the hydrolysis temperature was 55 °C, which was 23.63%. Conclusion This study optimize the production process of peptone from yak milk casein, and provided support for the further development and utilization of yak milk casein.
PDF
234
107
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
