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Objective To achieve rapid and non-destructive detection of lactic acid and total acidity during the fermentation process of sour meat. Methods Utilizing hyperspectral imaging technology, spectral reflectance images in the range of 408 to 1049 nm were collected to obtain spectral information of sour meat at different fermentation stages. After extracting the reflectance spectra from the regions of interest in the images, the kennard-stone algorithm (KS) was employed to divide the data into training and testing sets. The raw data underwent preprocessing through standard normal variate transformation (SNV) and multivariate scatter correction (MSC), followed by model establishment using partial least squares regression (PLSR). Feature wavelengths were extracted using the successive projection algorithm (SPA), competitive adaptive reweighted sampling (CARS), and uninformative variable elimination (UVE). Models were developed based on PLSR and compared with full-wavelength prediction models. Results The optimal prediction model for lactic acid in sour meat was SNV-CARS-PLSR, with a coefficient of determination (R2) of 0.9113 and a root mean square error of cross-validation (RMSECV) of 0.7236 for the training set, while the testing set yielded an R2 of 0.9104 and RMSECV of 0.7342. The MSC-CARS-PLSR model for total acidity demonstrated the best predictive performance, with training set R2 and RMSECV values of 0.9307 and 0.6782, respectively, and prediction set R2 and RMSECV values of 0.8682 and 0.6907. Conclusion The models constructed using hyperspectral imaging technology have the potential to enable rapid and non-destructive detection of lactic acid and total acidity in sour meat, indicating significant application value.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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