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Study on the changing rule of plasmin activity in processing of ultra high temperature milk
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Yu-Xin ZHANG1, Kai ZHAO2, Jie MIN1, Kai-Ru HE1, Yun-Jiang LI2, Min WANG2, Ri-Na WU1, Fei-Yu AN1, San-Jun ZHAO2, *, Jun-Rui WU1, *
Journal of Food Safety & Quality | 2025, 16(2) : 63 - 71
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Journal of Food Safety & Quality | 2025, 16(2): 63-71
Special Topic: Dairy Processing and Quality Control
Study on the changing rule of plasmin activity in processing of ultra high temperature milk
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Yu-Xin ZHANG1, Kai ZHAO2, Jie MIN1, Kai-Ru HE1, Yun-Jiang LI2, Min WANG2, Ri-Na WU1, Fei-Yu AN1, San-Jun ZHAO2, *, Jun-Rui WU1, *
Affiliations
  • 1. Shenyang Agricultural University, School of Food Science, Liaoning Food Fermentation Technology Engineering Research Center, Shenyang Key Laboratory of Microbial Fermentation Technology Innovation, Shenyang 110866, China
  • 2. Inner Mongolia Mengniu Dairy (Group) Co., Ltd., Huhhot 010010, China
Published: 2025-01-25 doi: 10.19812/j.cnki.jfsq11-5956/ts.20241031005
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Objective To investigate the key factors that may influence plasmin activity during the production of ultra high temperature (UHT) milk. Methods An enzyme-linked immunosorbent assay (ELISA) was employed to assess and analyze the effects of various factors on plasmin activity throughout the UHT milk production process. The specific factors examined included the degree of mastitis in cows, somatic cell count in milk, vaccination status, changes in feeding conditions, centrifugation processes, pasteurization preheating conditions, microfiltration techniques, and UHT preheating conditions. Results At the raw milk stage, a significant positive correlation was observed between plasmin activity levels in bovine milk and both the severity of bovine mastitis and somatic cell counts. However, no significant correlation was found regarding vaccination status or changes in feed type. During UHT milk production, centrifugal sterilization resulted in a slight increase in plasmin activity; pasteurization preheating at 55 ℃ proved most effective at inhibiting this increase. Microfiltration reduced plasmin activity by approximately 10%. Additionally, varying UHT preheating conditions led to an overall increase in enzyme activity; however, under treatment at 90 ℃ for 5 seconds, a condition yielding only a minimal increase of 1.52%, the rise remained relatively low. Conclusion For pasture, it is essential to focus on managing cows with mastitis and monitoring those milk with abnormal somatic cell counts. At the processing stage, implementing appropriate preheating conditions for pasteurization and UHT can effectively inhibit increases in plasmin activity within milk. Furthermore, incorporating microfiltration can serve as an additional strategy to reduce such enzymatic activities. This study provides a scientific foundation for further research aimed at controlling elevations of plasmin activity in UHT milk.

ultra high temperature milk  /  plasmin  /  enzyme-linked immunosorbent assay
Yu-Xin ZHANG, Kai ZHAO, Jie MIN, Kai-Ru HE, Yun-Jiang LI, Min WANG, Ri-Na WU, Fei-Yu AN, San-Jun ZHAO, Jun-Rui WU. Study on the changing rule of plasmin activity in processing of ultra high temperature milk[J]. Journal of Food Safety & Quality, 2025 , 16 (2) : 63 -71 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20241031005
Year 2025 volume 16 Issue 2
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20241031005
  • Receive Date:2024-10-31
  • Online Date:2025-07-21
  • Published:2025-01-25
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  • Received:2024-10-31
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Affiliations
    1. Shenyang Agricultural University, School of Food Science, Liaoning Food Fermentation Technology Engineering Research Center, Shenyang Key Laboratory of Microbial Fermentation Technology Innovation, Shenyang 110866, China
    2. Inner Mongolia Mengniu Dairy (Group) Co., Ltd., Huhhot 010010, China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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