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Objective To study the effect of n-3 polyunsaturated phosphatidylserine on the anti-apoptotic ability of rat hippocampal neuron cells (RHNC). Methods An apoptosis model using RHNC as the target and phorbol acetate (PMA) as the inducer was established. Using docosahexaenoic acid (DHA)/eicosapentaenoic acid (EPA) phosphatidylserine (DHA/EPA-PS) as a protective agent, the morphology of cells at different stages of apoptosis was observed under a laser confocal microscope after staining with acridine orange. The effect of n-3 polyunsaturated phosphatidylserine, represented by DHA/EPA-PS, on the anti-apoptotic ability of RHNC was studied at the cellular level through the Annexin V-FITC/PI double staining experiment. Results An RHNC apoptosis model with a PMA concentration of 10.0 mg/L and an action time of 24 hours was established. The 15.0 mg/L DHA/EPA-PS showed the best cell pre protection effect, with an apoptosis rate of 11.46%±4.11%. Early and mid-stage apoptotic cells were fewer and did not produce late stage apoptotic cells. Cells pre protected with 15 mg/L DHA/EPA-PS exhibited intact morphology and nuclear membrane, and their protrusions were not affected by PMA. Conclusion n-3 polyunsaturated phosphatidylserine, represented by DHA/EPA-PS, has certain anti-apoptotic ability against RHNC. The study provides good data support for future research on the biological activity of phosphatidylserine.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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