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Research on fungal culturomics methods in food based on 3 kinds of different culture media
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Yuan YUE1, *, Meng-Shi ZHOU2, Xiu-Juan FENG1, Shu-Zhen HE1, Yang SU1, Wei HE1, Rui LI2
Journal of Food Safety & Quality | 2025, 16(2) : 302 - 308
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Journal of Food Safety & Quality | 2025, 16(2): 302-308
• Food Analysis and Detection •
Research on fungal culturomics methods in food based on 3 kinds of different culture media
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Yuan YUE1, *, Meng-Shi ZHOU2, Xiu-Juan FENG1, Shu-Zhen HE1, Yang SU1, Wei HE1, Rui LI2
Affiliations
  • 1. Ningxia Hui Autonomous Region Food Testing Institute, Key Laboratory of Quality Safety of Chinese Wolfberry and Wine, State Administration for Market Regulation, National Wine Product Quality Inspection and Testing Centre (Ningxia), Yinchuan 750002, China
  • 2. Xi'an Biorealcoming Biomedical Center Co., Ltd., Xi'an 710000, China)
Published: 2025-01-25 doi: 10.19812/j.cnki.jfsq11-5956/ts.20240624001
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Objective To establish a solution for fungal culturomics in food by comparing the performance of 3 fungal culture media and optimising fungal culturomics techniques. Methods The 24 food samples and 2 proficiency test samples were tested using commonly used fungal culture media for food testing, including potato dextrose agar (PDA), rose Bengal agar, and a novel Pan Fungi medium (PF). The cultured fungi were isolated and identified using matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Results Yeast phase fungi could grow well on all 3 kinds of media, and the colony growth rate, colony morphology size and colony numbers had no significant difference. Filamentous fungi were able to grow well on the 3 kinds of medias, but growth rate and colony morphology were significantly different. There were 12 kinds of fungal species identified in the 24 samples, including 2 kinds of yeast phase fungi and 10 kinds of filamentous fungi. Conclusion With excellent accuracy and stability and self-limited growth of cultured colonies, PF fungal medium is favourable for isolation and identification of single colonies, and can be used as a supplement to the commonly used fungal medium for fungal culturomics studies in food.

fungi  /  culturomics  /  isolation and culture
Yuan YUE, Meng-Shi ZHOU, Xiu-Juan FENG, Shu-Zhen HE, Yang SU, Wei HE, Rui LI. Research on fungal culturomics methods in food based on 3 kinds of different culture media[J]. Journal of Food Safety & Quality, 2025 , 16 (2) : 302 -308 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20240624001
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doi: 10.19812/j.cnki.jfsq11-5956/ts.20240624001
  • Receive Date：2024-06-24
  • Online Date：2025-07-21
  • Published：2025-01-25
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  • Received：2024-06-24
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Affiliations
    1. Ningxia Hui Autonomous Region Food Testing Institute, Key Laboratory of Quality Safety of Chinese Wolfberry and Wine, State Administration for Market Regulation, National Wine Product Quality Inspection and Testing Centre (Ningxia), Yinchuan 750002, China
    2. Xi'an Biorealcoming Biomedical Center Co., Ltd., Xi'an 710000, China)
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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