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Objective To establish a rapid method for the determination of 5 kinds of nucleotides (cytidine-5’-monophosphate, adenosine-5’-monophosphate, uridine-5’-monophosphate, guanosine-5’-monophosphate and inosine-5’-monophosphate) in infant formulas and dairy products is developed based on mixed-mode hydrophilic interaction/ion exchange chromatography-ultraviolet detection (HILIC/IEX-UV). Methods A zwitterionic hydrophilic interaction chromatography column (Alphasil S-Xion) was used, with mobile phase consisting of 5 mmol/L KH2PO4 (pH adjusted to 3.0 with H3PO4)-acetonitrile (70:30, V:V) to detect nucleotides in infant foods and dairy products. Results The five nucleotides were baseline separated within 10 min, demonstrating efficient chromatographic performance. They exhibited good linearity within the range of 2-50 μg/mL, with limits of detection (LODs) between 0.19 and 0.66 mg/kg, and limits of quantification (LOQs) ranging from 0.62 to 2.19 mg/kg. At spiked levels of 50, 100 and 200 mg/kg, the intraday recoveries ranged from 96.6% to 108.9%, with relative standard deviations (RSDs) of 1.5%-6.3%, while the interday recoveries were between 101.0% and 108.0%, with RSDs of 2.4%-7.3%. Conclusions Compared with the traditional reversed-phase ion-pair chromatography, this method has significant advantages: Effectively reducing the matrix interference, shortening the analysis time, improving the detection efficiency, and especially improving the quantitative accuracy of cytidine-5’-monophosphate. The proposed method is rapid, accurate, and anti-interference, which provides technical support for the efficient analysis of nucleotides in infant formulas and dairy products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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