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Objective To establish an analytical method for determining γ-amino butyric acid content in foods of plant origin by high performance liquid chromatography. Method Foods samples were pre-column derivatized with 4-dimethylamino-azobenzene 4-sulfonyl chloride, orthogonal experiments were designed and methodological verification was carried out based on extraction solvent, solid-liquid ratio, extraction method and extraction time. The results of γ-amino butyric acid were analyzed by detecting different plant-derived foods and comparing with instruments. Results The results showed that the optimal conditions were determined as 60% ethanol solution, which was extracted by water bath (40 ℃) or ultrasonic for 30 min at 1:25 (g:mL) solid-liquid ratio. The separation of γ-amino butyric acid was achieved on C18 column (250 mm×4.6 mm, 5 μm) using acetonitrile+sodium acetate solution (20:80, V:V) as the mobile phase at a flow rate of 1.0 mL/min. The absorption wavelength of γ-amino butyric acid was 436 nm and column temperature was set at 30 ℃, respectively. The linear range of γ-amino butyric acid was 1.092-131.064 μg/mL. The relative standard deviation of γ-amino butyric acid was all less than 5.0% in pitaya (white heart), tomato, and malt. The average recovery rate of γ-amino butyric acid was between 95.80%-104.36%, indicating that the method had good precisions and high recoveries. Conclusion The method established in this study has good repeatability and high accuracy, and can accurately quantify the content of γ-amino butyric acid in foods of plant origin.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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