Objective To study investigates the feasibility of accurately tracing the origin of Fragariaananassa Duch. using stable isotopes and regional characteristics of multiple elements. Methods Using stable isotope ratio mass spectrometry technology and inductively coupled plasma mass spectrometry, the stable isotope ratios of carbon (13C/12C), nitrogen (15N/14N), hydrogen (2H/1H), and oxygen (18O/16O) and the content of 18 kinds of elements in Fragariaananassa Duch. samples was tested. The characteristic indicators of Fragariaananassa Duch. from different origins were analyzed, and stepwise discriminant analysis was used to establish 3 kinds of origin discrimination models for stable isotopes, mineral elements, and stable isotopes and mineral elements. The traceability of Fragariaananassa Duch. origins in Changping, Shuangliu, and Dandong was carried out. Results The correct discrimination rates of cross testing of 3 kinds of models were 88.4%, 90.7%, and 100.0%, respectively. Conclusion Research has shown that using a single technology for facility Fragariaananassa Duch. origin tracing and identification is not ideal, while simultaneously using stable isotope and elemental technologies for tracing can accurately distinguish the origin and provide a methodological basis for the feasibility of facility grown product origin tracing.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |