PDF
Full
Outline
Objective To establish a method for the determination of 8 kinds of fungal toxins in rice noodles with liquid-liquid dispersion extraction and multifunctional purification by high performance liquid chromatography-tandem mass spectrometry. Methods Rice noodle samples were added with isotopic internal standards, and extracted by shaking with acetonitrile water (80:20, V:V) for 25 min. After liquid-liquid dispersion extraction, a multifunctional purification column was used for purification. The 8 kinds of target compounds were separated on HSS T3 column, with acetonitrile-water (0.1% formic acid) used as the mobile phase for gradient elution, and detected under positive and negative ionization and multiple reaction monitoring mode, determined with retention time and characteristic ion pairs, quantified with internal standard method. Results This method had a good linear relationship in detecting 8 kinds of fungal toxins within the corresponding range. The correlation coefficients (r) of the target compounds were 0.9954-0.9998. The limits of detection were 0.10-10.00 μg/kg, and the limits of quantification were 0.30-30.00 μg/kg. The average recoveries of rice noodle samples at low, middle and high standard additions were 75.8%-112.0%, and the relative standard deviations were 2.9%-5.9%. The detection and analysis of 8 kinds of fungal toxins in 21 kinds of rice noodle samples in the market showed that all the toxins were detected except HT-2. Conclusion The pretreatment of sample is simple, the method is accurate and sensitive, which can be used to determine the content of 8 kinds of fungal toxins in rice noodles.
PDF
334
129
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
