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Objective To explore the effects of common food preservatives on the abundance and flora structure of antimicrobial-resistant bacteria in Crassostrea gigas. Methods The control group was refrigerated at 4 ℃, and the experimental group was refrigerated at 4 ℃ after soaking with potassium sorbate, chitosan, Nisin and ɛ-polylysine, respectively. The change of antimicrobial-resistant bacteria content in Crassostrea gigas treated with different food preservatives was analyzed by antimicrobial-resistant plate counting method. The structural changes of antimicrobial-resistant bacteria were analyzed by high-throughput sequencing of 16S rRNA amplicon. Results Potassium sorbate and chitosan could significantly reduce the number of resistant bacteria of single and multiple antimicrobials; Nisin significantly reduced the number of resistant bacteria to co-trimoxazole, chloramphenicol, gentamicin, and bacteria resistant to 2, 3, or 4 antimicrobials simultaneously; ε-polylysine significantly decreased the number of ciprofloxacin resistant bacteria, co-trimoxazole resistant bacteria, chloramphenicol resistant bacteria, gentamicin resistant bacteria and resistant bacteria resistant to 3 or 4 kinds of antimicrobials at the same time. Potassium sorbate and chitosan had a significant effect on the composition of tetracycline and ciprofloxacin resistant bacteria, and Nisin and ε-polysine had a significant effect on the composition of co-trimoxazole resistant bacteria. Conclusion The research results show that the content of antimicrobial-resistant bacteria in Crassostrea gigas can be controlled by applying appropriate food preservatives, food preservatives can affect the composition of culturable antimicrobial-resistant bacteria in Crassostrea gigas and different preservatives can inhibit specific antimicrobial-resistant bacteria, which provides a new idea for the establishment of antimicrobial-resistant bacteria control techniques in Crassostrea gigas.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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