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Objective To develop an ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of pentachlorophenol sodium residues in aquatic products. Methods The sample preparation involved extraction with 80% acetonitrile in water containing 5% triethylamine, followed by purification using Waters Oasis MAX solid-phase extraction cartridges. After elution with a 4% formic acid in methanol solution, the pentachlorophenol sodium was analyzed by UPLC-MS/MS using a Waters XBridge C18 column (100 mm × 2.10 mm, 3.50 µm). The mobile phases consisted of A: 0.1% formic acid in water (containing 5 mmol/L ammonium acetate) and B: acetonitrile. Quantification was carried out using the external standard method. The recovery of pentachlorophenol sodium in the muscle of Ctenopharyngodon idella and the eatable part of Eriocheir sinensis matrices was examined. Results The linear range of the pentachlorophenol sodium residue detection method in Ctenopharyngodon idella and Eriocheir sinensis was 0.2 to 8.0 ng/mL (r2>0.999). The limit of detection was 0.5 μg/kg and limit of quantification was 1.0 μg/kg, respectively. The recoveries at spiking levels of 1.0, 2.0, and 10 μg/kg were within the range of 77.0% to 105%. Intra-assay relative standard deviations ranged from 2.51% to 7.62% (n=6), and inter-assay relative standard deviations ranged from 3.36% to 6.48% (n=3). Conclusion The developed UPLC-MS/MS method for the determination of pentachlorophenol sodium residues in Ctenopharyngodon idella and Eriocheir sinensis matrices is highly sensitive, accurate, and precise, with good repeatability and reproducibility, and is therefore suitable for the detection of pentachlorophenol sodium residues in aquatic products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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