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Development and application of colloidal gold enzyme-linked immunochromatographic test strip for amanitin detection
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Jian-Lin TAN1, Zhen-Hua PENG1, Hao SUN1, Li YANG1, Ya-Qin WANG1, Shu-Bing FANG2, Yan-Bin XIN1, Xue-Tao MA1, Zhi-Rui NIU1, *
Journal of Food Safety & Quality | 2025, 16(8) : 69 - 75
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Journal of Food Safety & Quality | 2025, 16(8): 69-75
• Special Topic: Detection and Prevention and Control of Biotoxins in Food •
Development and application of colloidal gold enzyme-linked immunochromatographic test strip for amanitin detection
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Jian-Lin TAN1, Zhen-Hua PENG1, Hao SUN1, Li YANG1, Ya-Qin WANG1, Shu-Bing FANG2, Yan-Bin XIN1, Xue-Tao MA1, Zhi-Rui NIU1, *
Affiliations
  • 1. Yunnan Institute of Product Quality Supervision and Inspection, National Tropical Agricultural By-products Quality Inspection and Testing Center, Kunming 650223, China
  • 2. Qingdao Pribolab Bioengineering Co., Ltd., Qingdao 266001, China
Published: 2025-04-25 doi: 10.19812/j.cnki.jfsq11-5956/ts.20241028010
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Objective To develop an enzyme-linked immunochromatographic test strip for the determination of amanita toxin in mushroom based on immunochromatography. Methods N-hydroxysuccinimide (NHS) and N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) were used as activators, 6-aminohexanoic acid was introduced to obtain amanitin peptide hapten, and further conjugated with keyhole limpet hemocyanin (KLH) and bovine serum albumin (BSA) to prepare immunogen and coating antigen. Then, the immunogen was used to immunize Balb/c mice to prepare monoclonal antibodies. The colloidal gold immunochromatographic test strip for amanitin was successfully developed by preparing colloidal gold and gold labeled antibody, optimizing the concentration of coating antigen, secondary antibody and extracting solution, and then its cross reactivity, stability, repeatability, and accuracy were evaluated. Results The limits of detection of α-amatoxin, β-amatoxin, and γ-amatoxin peptide were 100, 20 and 50 μg/kg, respectively. The sensitivity was 100%, the false positive rate and false negative rate were 0%, and the results were consistent with the existing instrumental methods without cross reaction with other mushroom toxins. Conclusion The test strip has the characteristics of simple operation, high repeatability and good stability, and is suitable for on-sites creening and rapid detection of amanitin in mushrooms.

amanitin  /  colloidal gold  /  immunochromatographic test strip  /  mushroom
Jian-Lin TAN, Zhen-Hua PENG, Hao SUN, Li YANG, Ya-Qin WANG, Shu-Bing FANG, Yan-Bin XIN, Xue-Tao MA, Zhi-Rui NIU. Development and application of colloidal gold enzyme-linked immunochromatographic test strip for amanitin detection[J]. Journal of Food Safety & Quality, 2025 , 16 (8) : 69 -75 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20241028010
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Year 2025 volume 16 Issue 8
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20241028010
  • Receive Date：2024-10-28
  • Online Date：2025-07-19
  • Published：2025-04-25
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History
  • Received：2024-10-28
Funding
Affiliations
    1. Yunnan Institute of Product Quality Supervision and Inspection, National Tropical Agricultural By-products Quality Inspection and Testing Center, Kunming 650223, China
    2. Qingdao Pribolab Bioengineering Co., Ltd., Qingdao 266001, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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