Objective To study the accuracy and feasibility of colloidal gold immunochromatography for the detection of animal-derived components in meat products. Methods The rapid detection kit was used to detect the animal-derived components in meat products. The sensitivity, specificity, adulteration simulation, false positive rate and false negative rate of 8 kinds of animal-derived components were tested, and quantitative real-time polymerase chain reaction (QPCR) was used as a reference. Results The limits of detection of the rapid detection kit could reach 0.1% for cattle, chicken, duck and goose, and 0.01% for pig, sheep, horse and donkey. The limits of detection of pig, cattle, sheep, chicken, duck, goose, horse and donkey in the adulterated simulation could reach 0.5%, and there was no cross-reaction with other non-species channel detection, and the specificity was good. Conclusion The colloidal gold immunochromatography method established in this study is suitable for the rapid screening and detection of animal origin in meat products.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |