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Research on the detection of neonicotinoid pesticides in Musa nana Lour. based on competitive inhibition immunochromatographic technology
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Xiao-Yu GUO1, 2, Ying WEI1, 2, Hong CHANG1, 2, Man-Tun GU1, 2, Ying ZHOU1, 2, Rui KANG1, 2, *
Journal of Food Safety & Quality | 2025, 16(7) : 78 - 86
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Journal of Food Safety & Quality | 2025, 16(7): 78-86
• Special Topic: Analysis and Monitoring of Toxic and Harmful Substances in Food •
Research on the detection of neonicotinoid pesticides in Musa nana Lour. based on competitive inhibition immunochromatographic technology
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Xiao-Yu GUO1, 2, Ying WEI1, 2, Hong CHANG1, 2, Man-Tun GU1, 2, Ying ZHOU1, 2, Rui KANG1, 2, *
Affiliations
  • 1. Institute of Food Testing, Hainan Academy of Inspection and Testing, Haikou 570314, China
  • 2. Key Laboratory of Tropical Fruits and Vegetables Quality and Safety, State Administration for Market Regulation, Haikou 570314, China
Published: 2025-04-15 doi: 10.19812/j.cnki.jfsq11-5956/ts.20241223006
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Objective To establish a rapid competitive inhibition immunochromatographic detection method for the simultaneous determination of 3 kinds of neonicotinoid pesticides with a high non-compliance rate in Musa nana Lour.. Methods The samples were extracted using a solution composed of 20% methanol, 0.05% Tween, and 0.02 mol/L phosphate-buffered saline (PBS). After centrifugation at 8000 r/min for 5 min, 100 μL of the supernatant was taken and diluted according to specific dilution ratios. Subsequently, 100 μL of the diluted sample solution was added to the sample well (S). The results were read after 8 min reaction period. Results The limits of detection for imidacloprid, clothianidin, and thiamethoxam were 0.050, 0.020 and 0.002 mg/kg, respectively, with corresponding sample dilution factors of 2-fold, no dilution, and 5-fold. Upon evaluation, the relative accuracies were determined to be 96.7%, 98.3% and 100.0%, respectively, which were in agreement with the results obtained by the liquid chromatography-mass spectrometry method for the detection of these pesticides in Musa nana Lour.. Conclusion This rapid detection method exhibits characteristics such as simplicity in operation, rapidity in detection, and portability of the instrument, rendering it suitable for on-site and rapid screening of pesticide residues in Musa nana Lour..

immunochromatography technology  /  Musa nana Lour.  /  neonicotinoid pesticide  /  rapid detection
Xiao-Yu GUO, Ying WEI, Hong CHANG, Man-Tun GU, Ying ZHOU, Rui KANG. Research on the detection of neonicotinoid pesticides in Musa nana Lour. based on competitive inhibition immunochromatographic technology[J]. Journal of Food Safety & Quality, 2025 , 16 (7) : 78 -86 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20241223006
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Year 2025 volume 16 Issue 7
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20241223006
  • Receive Date：2024-12-23
  • Online Date：2025-07-19
  • Published：2025-04-15
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History
  • Received：2024-12-23
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Affiliations
    1. Institute of Food Testing, Hainan Academy of Inspection and Testing, Haikou 570314, China
    2. Key Laboratory of Tropical Fruits and Vegetables Quality and Safety, State Administration for Market Regulation, Haikou 570314, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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