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Objective To develop a nutritional beverage rich in Auricularia auricula polysaccharide and polypeptide, explore its biological activity in health care. Methods Polysaccharides and polypeptide were extracted from high-quality Auricularia auricula by enzymatic hydrolysis. The regulation of blood pressure and antioxidant activity were evaluated by in vitro experiments, and the content was determined. With sensory evaluation as the key index, the best formula was finally determined by screening sweeteners, sour agents and stabilizers. Results The optimum formula was as follows: The addition amount of Auricularia auricular polypeptide extract was 0.1 %, the addition amount of Auricularia auricular polysaccharide extract was 0.5%, the addition amount of citric acid was 0.04%, the addition amount of sucrose was 6%, the polysaccharide content was 528.1 mg/100 mL, the polypeptide content was 177.9 mg/100 mL, and the angiotensin converting enzyme (ACE) inhibition rate was (58.61±0.72)%. The scavenging rate of 1,1-diphenyl-2-picrylhydrazyl (DPPH) was (68.22±1.06)%, the scavenging ability of hydroxyl radical was (70.31±0.68)%, and the scavenging ability of superoxide anion was (61.70±0.37)%, which indicated that the beverage had good health care effect in regulating blood pressure, blood glucose and antioxidant. Conclusion The beverage not only retains the traditional health benefits of Auricularia auricula, but also optimizes the taste through scientific matching and advanced processing technology to obtain higher content of polysaccharides and peptides. The verification results of its biological activity provide a scientific basis for the commercial production of Auricularia auricula beverage in the field of health care.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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