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Simultaneous determination of 9 kinds of components in Andrographis paniculata by one-point multi-component method based on ultra performance liquid chromatography-tandem mass spectrometry
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Shao-Hua CHEN1, Xi HUANG2, Kai-Ke YU2, Fei-Ru ZHU2, Yan ZHENG2, Feng LI2, Li-Li LIU2, You-Fang YE2, Zi-Xia HUANG2, Guo-Hua LIANG2, *
Journal of Food Safety & Quality | 2025, 16(6) : 122 - 131
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Journal of Food Safety & Quality | 2025, 16(6): 122-131
• Special Topic: Research and Detection of Pesticide and Veterinary Drug Residue •
Simultaneous determination of 9 kinds of components in Andrographis paniculata by one-point multi-component method based on ultra performance liquid chromatography-tandem mass spectrometry
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Shao-Hua CHEN1, Xi HUANG2, Kai-Ke YU2, Fei-Ru ZHU2, Yan ZHENG2, Feng LI2, Li-Li LIU2, You-Fang YE2, Zi-Xia HUANG2, Guo-Hua LIANG2, *
Affiliations
  • 1. Beihai Food and Drug Safety Certification and Evaluation Center, Beihai 536000, China
  • 2. Beihai Public Inspection and Testing Center, Beihai 536000, China
Published: 2025-03-25 doi: 10.19812/j.cnki.jfsq11-5956/ts.20250109007
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Objective To establish a quantitative analysis of multi-components by single marker (QAMS) method for the simultaneous determination of 9 kinds of components in Andrographis paniculata. Method The relative calibration factors of andrographolide with forsythitin, oleanolic acid, caffeic acid, andrographolide, dehydrated andrographolide, andrographolide, deoxyandrographolide, dehydrated andrographolide half succinate and sodium bisulfite were established by ultra performance liquid chromatographolide-tandem mass spectrometry. The relative correction factor was used to calculate the content of each component, and the calculated results of QAMS method were compared with the measured values of external standard method. Results The relative correction factors showed good reproducibility, and there was no significant difference between the results obtained by the QAMS method and the external standard method. Conclusion The QAMS method is suitable for the simultaneous determination of 9 kinds of components in Andrographis paniculata, ensuring the reliability of quality.

Andrographis paniculata  /  ultra performance liquid chromatography-tandem mass spectrometry  /  one-point multi-component method
Shao-Hua CHEN, Xi HUANG, Kai-Ke YU, Fei-Ru ZHU, Yan ZHENG, Feng LI, Li-Li LIU, You-Fang YE, Zi-Xia HUANG, Guo-Hua LIANG. Simultaneous determination of 9 kinds of components in Andrographis paniculata by one-point multi-component method based on ultra performance liquid chromatography-tandem mass spectrometry[J]. Journal of Food Safety & Quality, 2025 , 16 (6) : 122 -131 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20250109007
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Year 2025 volume 16 Issue 6
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20250109007
  • Receive Date：2025-01-09
  • Online Date：2025-07-19
  • Published：2025-03-25
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  • Received：2025-01-09
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Affiliations
    1. Beihai Food and Drug Safety Certification and Evaluation Center, Beihai 536000, China
    2. Beihai Public Inspection and Testing Center, Beihai 536000, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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