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Objective To optimize the extraction process of Chaenomeles sinensis polyphenols and study their antioxidant activity. Methods The extraction process of Chaenomeles sinensis polyphenols was optimized through single factor and orthogonal experiments; the in vitro experiments were simultaneously conducted to investigate the total reducing ability, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability, and hydroxyl radical scavenging ability of Chaenomeles sinensis polyphenols. Results The optimal process for extracting polyphenols from Chaenomeles sinensis was: Ethanol volume fraction of 60%, solid-liquid ratio of 1:25 (g/mL), ultrasonic temperature of 55 ℃ and ultrasonic time of 90 min. Under these conditions, the polyphenol extraction amount was 2.22 mg/g; at a sample concentration of 1.0 mg/mL, the total reducing ability, DPPH radical scavenging rate and hydroxyl radical scavenging rate of Chaenomeles sinensis polyphenols were determined to be 2.41±0.02, 89.33%±0.24%, and 81.30%±1.27%, respectively, all higher than vitamin C at the same concentration (2.29±0.08, 86.05%±0.39%, 80.94%±0.41%), indicating that Chaenomeles sinensis polyphenols had good antioxidant activity and promising application prospects. Conclusion This study provides a reference for further research and application of polyphenols in Chaenomeles sinensis, offers new ideas for the diversified and high-value utilization of Chaenomeles sinensis resources, and provides theoretical basis for the deep processing of Chaenomeles sinensis resources and rational dietary selection.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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