PDF
Full
Outline
Objective To establish a method for the simultaneous determination of functional components in extracts from 7 kinds of medicinal and food homologous substances: Pueraria lobata, Sophora japonica, Lonicera japonica, Cistanche deserticola, Siraitia grosvenorii, Panax ginseng and Hovenia dulcis by high performance liquid chromatography (HPLC). Methods The analysis was conducted using an Agilent Eclipse XDB-C18 column (4.6 mm× 250 mm, 5 μm) with a mobile phase gradient elution of acetonitrile-0.1% phosphoric acid at a flow rate of 0.6 mL/min, a column temperature of 30 ℃, a detection wavelength of 210 nm, and an injection volume of 5 μL. This method was employed to quantify the content of puerarin, rutin, chlorogenic acid, echinacoside, verbascoside, mogroside V, ginsenoside Re and dihydromyricetin. Results The results demonstrated that 8 kinds of functional components exhibited excellent linear relationships within their respective concentration ranges, with correlation coefficients (r²) exceeding 0.999. The limits of detection ranged from 0.02 to 1.88 mg/L, and the limits of quantification ranged from 0.08 to 3.62 mg/L. The precision experiment results showed that the relative standard deviation (RSD) was less than 3%, the average recoveries of spiked samples ranged from 95.49% to 109.87%. Conclusion This method is simple, rapid and highly accurate, making it suitable for the qualitative and quantitative analysis of the functional components in the aforementioned 7 kinds of medicinal and food homologous substances.
PDF
313
121
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
