PDF
Full
Outline
Objective To establish a method for simultaneous determination the content of 4 kinds of human milk oligosaccharides [2'--fucosyllactose (2'-FL), 3'-sialic acid (3'-SL), 6'-sialic acid (6'-SL) and lactose-N-neotetraose (LNnT)] in milk powder by liquid chromatography-fluorescence method. Methods The samples were dissolved in water, enzymolized with amyloglucosidase or β-galactosidase, derived with 2-aminobenzamide (2-AB) and 2-methylpyridine borane (2-PB), and separated by amide bonding column, detected by fluorescence detector, and quantitated by internal standard methods. Results The 4 kinds of milk oligosaccharides had a good linear relationship in the concentration range of 10-600 μg/mL, and the correlation coefficients (r2) were more than 0.999. The limits of detection and quantification of 4 kinds of milk oligosaccharides were 0.94-2.31 mg/100 g and 3.12-7.69 mg/100 g, respectively; the recovery rates of 4 kinds of milk oligosaccharides were 97.7%-101.5%, and the relative standard deviations (RSD) (n=7) were 0.53%-3.09%. Conclusion This method does not have high requirements for people, equipments and environment for determining the content of 4 kinds of milk oligosaccharides in milk powder. The pre-treatment operation is simple, and it has good accuracy and precision. It can provide a reference for the quality control method of human milk oligosaccharides in milk powder.
PDF
273
130
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
