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Objective To screen a strain that can antagonize the growth of Aspergillus flavus and to identify the strain, study its application and analysis its genetic preliminarily. Methods The microorganisms in the soil of peanut field in Feixian County were used as the research object, and the effective strains were obtained through the screening of Aspergillus flavus standoff test; the effective strains species were determined through morphological observation and 16S rRNA gene analysis and identification; the peanut pods and peanut kernels were infested with the suspension of Aspergillus flavus spores to validate the effective bacteria’s effect of antagonizing the growth of Aspergillus flavus; and the genome characteristics of the bacterial strains and the clusters of genes of secondary metabolites synthesis were analyzed to determine the basic characteristics of the effective bacterial strains. The genomic characteristics of the strain and the secondary metabolite synthesis gene cluster were analyzed to determine the basic characteristics of the effective strain. Results Effective strain G22, which could antagonize the growth of Aspergillus flavus, was obtained by screening. The strain G22 was identified as Burkholderia latens with 99.93% homology and named Burkholderia latens G22 by morphological characterization and gene homology analysis. The fermentation broth of the effective strain had a certain antagonistic effect on the growth of Aspergillus flavus on peanut pods and peanut seed kernels. The genome of the effective strain had 3 chromosomes and 1 plasmid, and the secondary metabolite synthesis gene cluster proved that the effective strain had antagonistic properties. Conclusion Strain G22 has good antagonistic effect on the growth of Aspergillus flavus. The application effect of strain G22 in peanut has important research significance in practice, which will lay a foundation for the further development of microbial fungicides for Aspergillus flavus.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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