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Determination of multiple components content in Tetrastigma hemsleyanum Diels et Gilg at different months and establishment of its fingerprint chromatograms
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Yu-Chen WANG1, 2, Yu CHEN1, 2, Ming-Zhu MA2, Yan SONG1, Jia-Xing WANG2, Ruan-Yi GUO3, Bin ZHANG1, Ji-Peng SUN2, *
Journal of Food Safety & Quality | 2025, 16(9) : 259 - 267
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Journal of Food Safety & Quality | 2025, 16(9): 259-267
• Food Analysis and Detection •
Determination of multiple components content in Tetrastigma hemsleyanum Diels et Gilg at different months and establishment of its fingerprint chromatograms
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Yu-Chen WANG1, 2, Yu CHEN1, 2, Ming-Zhu MA2, Yan SONG1, Jia-Xing WANG2, Ruan-Yi GUO3, Bin ZHANG1, Ji-Peng SUN2, *
Affiliations
  • 1. College of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, China
  • 2. Zhejiang Marine Development Research Institute, Zhoushan 316100, China
  • 3. Focus Plus Cosmetics (Shanghai) Co., Ltd, Shanghai 201802, China
Published: 2025-05-15 doi: 10.19812/j.cnki.jfsq11-5956/ts.20250212006
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Objective To investigate the main active components of different monthly Tetrastigma hemsleyanum Diels et Gilg (TDG) from Zhoushan City and establish the map of different monthly TDG. Methods The quality of different monthly TDG was evaluated by combining fingerprint similarity evaluation cluster analysis (CA), principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). An InterSustainSwift C18 chromatographic column (4.6 mm×250 mm, 5 μm) was used, with acetonitrile-0.1 formic acid aqueous solution as the mobile phase, gradient elution, flow rate of 0.8 mL/min, detection wavelength of 20 nm, column temperature of 30 ℃, and injection volume of 10 μL. Results The fingerprint similarity evaluation results of 12 batches of different monthly TDG showed that the similarity of different monthly TDG samples varied greatly; the 13 common were identified, and 3 common peaks were recognized; CA, PCA, and OPLS-DA analysis could all classify the 12 batches of different monthly TDG into 3 categories; and 2 differential quality markers, gallic acid and quercetin, were screened out by analyzing the variable importance in projection (VIP) values under OPLS-DA. Conclusion The high performance liquid chromatography (HPLC) fingerprint and multi-component content determination method established in this study is stable and reliable, and can provide a reference for quality control of Zhoushan TDG.

Tetrastigma hemsleyanum Diels et Gilg  /  different seasons  /  fingerprint chromatograms  /  content determination  /  quality control
Yu-Chen WANG, Yu CHEN, Ming-Zhu MA, Yan SONG, Jia-Xing WANG, Ruan-Yi GUO, Bin ZHANG, Ji-Peng SUN. Determination of multiple components content in Tetrastigma hemsleyanum Diels et Gilg at different months and establishment of its fingerprint chromatograms[J]. Journal of Food Safety & Quality, 2025 , 16 (9) : 259 -267 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20250212006
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Year 2025 volume 16 Issue 9
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20250212006
  • Receive Date：2025-02-12
  • Online Date：2025-07-17
  • Published：2025-05-15
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  • Received：2025-02-12
Funding
Affiliations
    1. College of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, China
    2. Zhejiang Marine Development Research Institute, Zhoushan 316100, China
    3. Focus Plus Cosmetics (Shanghai) Co., Ltd, Shanghai 201802, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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