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Objective To establish a method for the simultaneous detection of multiple amino acids in each part of pork by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods The samples were extracted by acidizing high-temperature hydrolysis, acidizing microwave hydrolysis and alkalization high-temperature hydrolysis, determined by HPLC-MS/MS, electrospray ionization in positive ion mode, multiple reaction monitoring (MRM), used formic acid solution-methanol acetonitrile (1:4, V:V) solution of formic acid as mobile phase with gradient elution. Results It was extracted by acidizing microwave hydrolysis with short time and high efficiency. The detection method had a good relationship in the linear range, r2 was between 0.9905 and 0.9956, the limits of detection were 0.012-0.133 ng/mL, the limits of quantitation were 0.038-0.444 ng/mL, the average recoveries were in the range 85.58% and 98.84%, the relative standard deviations (RSDs) were 0.68%-6.71%. Conclusion The method has simple process, good repeatability and stability, and can not only be used for simultaneous detection of multiple amino acids in each part of pork to achieve the purpose of high-throughput and rapid detection, but also can effectively monitor the adulteration behavior of pork products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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