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Objective To establish an analytical method for the determination of dehydroepiandrosterone (DHEA) content in variety of health food by liquid chromatography. Methods Tablets and capsules were extracted with acetonitrile:water (60:40, V:V); oral liquid was extracted with acetonitrile and added sodium chloride to salt out the targets from water; gel candy was dissolved in warm water first, and extracted with acetonitrile, after that ammonium sulfate was used to precipitate gelatin and made acetonitrile water stratification simultaneously. Finally, DHEA in health product was effectively extracted, then determined by liquid chromatography with diode array detection or liquid chromatography tandem mass spectrometry and quantified by external standard method. Results The established methods were effective in extracting DHEA from health foods, with limit of detection for solid or powder sample was 0.15 g/kg and for liquid was 1.5 mg/L and limit of quantification was 0.5 g/kg for solid or powder samples and 5 mg/L for liquid, respectively. The recovery of this method was 95.7%-104.0%, and the relative standard deviation was 1.10%-3.76%. It was found that the content of DHEA in imported dietary supplements was mostly consistent with the label, with a few samples not matching the label in real sample. Conclusion This method is sensitive, simple and convenient, and can be used not only to monitor the illegal addition of DHEA in domestic health foods, but also to evaluate the content of DHEA in cross-border dietary supplements.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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