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Objective To establish a method for the qualitative and quantitative determination of a new illegal additive in foods, vardenafil impurity 31 by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The UPLC-MS/MS was used to screen 92 kinds of substances of nafils illegally added in foods. The ultra performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) was used to analyze the structure of the test sample. After the structure was inferred, the standard substance was purchased and verified. The target substance in the test sample was confirmed to be vardenafil impurity 31. The multiple reaction monitoring (MRM) method was established by UPLC-MS/MS, which was suitable for the qualitative and quantitative determination of vardenafil impurity 31 in foods. Results The limit of detection of vardenafil impurity 31 was 5 μg/kg, and the limit of quantitation was 10 μg/kg. The linear relationship was good in the linear range of 0.1-50.0 ng/mL, the correlation coefficient (r) was 0.9976, the average recovery rate was 85.33%-89.41%, and the relative standard deviation (RSD) was less than 10%. Conclusion The established method is simple and accurate, and can be used for the qualitative and quantitative determination of the new illegal additive vardenafil impurity 31 in foods.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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