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Objective To optimize the ultrasonic-assisted extraction process of proanthocyanidins from Polygonum aviculare by response surface method, to obtain the best extraction conditions. Methods The main factors affecting the extraction amount were investigated through single-factor experiments. Based on the results of the single-factor experiments, the Box-Behnken design and response surface methodology were used to optimize the single factors, including the volume fraction of ethanol, ultrasonic time, and solid-liquid ratio, which had an impact on the extraction amount of proanthocyanidins from Polygonum aviculare. The optimal extraction process conditions were obtained. Results The optimized results of the optimal process conditions for ultrasonic-assisted extraction of proanthocyanidins from Polygonum aviculare were as follows: The ultrasonic time was 34 min, the volume fraction of ethanol was 65%, and the solid-liquid ratio was 1:46 (g:mL). Under these process conditions, the final extraction amount of proanthocyanidins reached 2.18%, which was consistent with the model validation value of 2.12%. Conclusion This study shows that the process condition parameters optimized by the response surface methodology are effective and feasible. The response surface methodology is successfully applied to optimize the extraction parameters of ultrasonic-assisted extraction of proanthocyanidins from Polygonum aviculare, providing a research basis for the later promotion and application of proanthocyanidins from Polygonum aviculare.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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