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Objective To optimize the fermentation process of pear residue soluble dietary fiber (PSDF) modified by Aspergillus niger and study its characteristics. Methods Using single factor experiments to optimize the inoculation amount, fermentation temperature, and fermentation time of Aspergillus niger, and determine the optimal fermentation process; determine the characteristics of the modified PSDF: The water holding capacity (WHC), oil holding capacity (OBC), fructose, glucose, arabinose, xylose and xylose content, 1,1-diphenyl-2- picrylhydrazyl radical (DPPH) free radical scavenging ability, 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) cationic radical scavenging ability, glucose adsorption capacity (GAC) and cholesterol adsorption capacity (CAC).Results By optimizing the fermentation process, the optimal inoculation amount, fermentation temperature, and fermentation time of Aspergillus niger were determined to be 8%, 36 ℃ and 7 d. Based on the optimal fermentation process, the characteristic analysis of PSDF was conducted: WHC was 15.89 g/g, and OBC was 4.02 g/g; the content of glucose and arabinose increases, while the content of fructose, xylose and xylose decreases; the DPPH radical scavenging ability and ABTS cationic radical scavenging ability were significantly improved; GAC increased to 0.77 mg/g, and CAC increased to 0.56 mg/g. Conclusion Modifying PSDF with suitable fermentation processes can help improve yield. The use of Aspergillus niger modified pear pomace dietary fiber effectively improve its physiological activity and health function.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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