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Outline
Total plate count in food is a key microbiological indicator for evaluating food hygiene quality,monitoring production processes and predicting product shelf life; the speed of testing determines the timeliness and accuracy of food safety regulation. Although the traditional plate count method GB 4789.2—2022 National food safety standard-Microbiological testing of foods-Determination of total colony count is considered the “gold standard” for total colony count testing, it is time-consuming and fails to meet the real-time testing requirements of the modern food industry for rapid verification. This paper reviewed the research progress in rapid total plate count detection technologies over the past decade, focused on the analysis and introduction of the principles, characteristics and optimization methods of various rapid detection platforms, such as flow cytometry, rapid test strips, adenosine triphosphate bioluminescence,electrical impedance, quantitative fluorescent polymerase chain reaction, sensors, hyperspectral imaging, dielectric spectroscopy, Soleris real-time photonic technology and surface-enhanced Raman spectroscopy. Based on this review,the paper analyzed and discussed the existing challenges and future trends of these technologies in terms of detection sensitivity, resistance to matrix interference, quantitative capabilities and on-site applicability, with the aim of providing a reference for in-depth research and industrial development of rapid food microbiological detection technologies.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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