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Identification and analysis of triterpenoids from Ganoderma lucidum by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry
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Le HUANG1, Hai-Yang QIU1, Ya-Hui SHANGGUAN1, Yan-Jian CHEN2, Qin-Ying LIU3, Qiao-Mei LU4, *, Li CHEN1, *
Journal of Food Safety & Quality | 2026, 17(15) : 115 - 123
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Journal of Food Safety & Quality | 2026, 17(15): 115-123
• Special Topic: Application of Modern Analysis Instrument in Food Detection •
Identification and analysis of triterpenoids from Ganoderma lucidum by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry
Full
Le HUANG1, Hai-Yang QIU1, Ya-Hui SHANGGUAN1, Yan-Jian CHEN2, Qin-Ying LIU3, Qiao-Mei LU4, *, Li CHEN1, *
Affiliations
  • 1.College of Biological Science and Engineering, Fuzhou University, Fuzhou 350116, China
  • 2.Fujian Xianzhilou Biological Technology Company Limited, Fuzhou 350108, China
  • 3.Fujian Provincial Key Laboratory of Tumor Biotherapy, Fujian Provincial Cancer Hospital, Fuzhou 350014, China
  • 4.Fujian College Association Instrumental Analysis Center of Fuzhou University, Fuzhou 350116, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20260520002
Outline
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Objective

To establish a rapid and high-throughput qualitative method for analysis of triterpenoids from Ganoderma lucidum by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).

Methods

Based on the advantages of UPLC-Q-TOF-MS with high separation efficiency and high resolution, the mass spectrometer detection conditions (including the ion detection mode, ion source temperature and collision energy) were optimized. The mass spectral fragmentation patterns and characteristic fragment ions of triterpenoids were summarized, and a standard spectral library of triterpenoids from Ganoderma lucidum was constructed. Meanwhile, sample spiking verification experiments were introduced. Combined with the retention time and accurate mass-to-charge ratio information of spiked samples, actual samples and reference standards, the rapid identification of triterpenoids was realized.

Results

After optimization, the mass spectrometry detection was employed electrospray ionization in negative-ion mode, with an ion source temperature of 450 ℃, a voltage of –4500 V and a collision energy of 45 V. Combined with standard substance comparison and triterpenoid cleavage rules, a total of 32 kinds of triterpenoid components and 5 kinds of unknown triterpenoid components were identified.

Conclusion

This analytical method has excellent separation performance, high sensitivity and strong specificity. It enables rapid qualitative identification and preliminary structural elucidation of triterpenoids from Ganoderma lucidum. It is applicable to the chemical composition characterization and quality control of Ganoderma lucidum and also provides a methodological reference for further research of triterpenoid active substances.

triterpenoids from Ganoderma lucidum  /  fragmentation rules  /  standard spectrum library  /  component identification
黄乐, 邱海洋, 上官雅慧, 陈言枧, 刘沁颖, 卢巧梅, 陈立. 超高效液相色谱-四极杆-飞行时间质谱法鉴定分析灵芝三萜类化合物. 食品安全质量检测学报, 2026 , 17 (15) : 115 -123 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20260520002
Le HUANG, Hai-Yang QIU, Ya-Hui SHANGGUAN, Yan-Jian CHEN, Qin-Ying LIU, Qiao-Mei LU, Li CHEN. Identification and analysis of triterpenoids from Ganoderma lucidum by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry[J]. Journal of Food Safety & Quality, 2026 , 17 (15) : 115 -123 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20260520002
Appendix
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Year 2026 volume 17 Issue 15
7
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20260520002
  • Receive Date：2026-05-20
  • Online Date：2026-11-05
Article Data
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History
  • Received：2026-05-20
Funding
Affiliations
    1.College of Biological Science and Engineering, Fuzhou University, Fuzhou 350116, China
    2.Fujian Xianzhilou Biological Technology Company Limited, Fuzhou 350108, China
    3.Fujian Provincial Key Laboratory of Tumor Biotherapy, Fujian Provincial Cancer Hospital, Fuzhou 350014, China
    4.Fujian College Association Instrumental Analysis Center of Fuzhou University, Fuzhou 350116, China
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https://castjournals.cast.org.cn/joweb/spaq/EN/10.19812/j.cnki.jfsq11-5956/ts.20260520002
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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