Full
Outline
To establish an accurate, sensitive and anti-interferential method for the determination of low-content total anthraquinones in health wine with complex matrix.
Based on the spectrophotometry method specified in 2020 Edition of the technical guiding principles for inspection and evaluation of physicochemical and hygienic indicators of health food, the sample pretreatment was optimized by adjusting sample weight, performing full-volume extraction, reducing the constant volume and adopting centrifugal purification to minimize matrix interference and enhance detection sensitivity. The optimized method was verified using the high performance liquid chromatography method under the item of Polygoni multiflori Radix in the 2025 edition of the Chinese pharmacopoeia as the reference method, and the consistency of the results was evaluated by Bland-Altman analysis.
The optimized method exhibited good linearity in the range of 0–0.0162 mg/mL with a determination coefficient of 0.99998.The limit of detection was 0.009 mg/100 g, and the limit of quantitation was 0.026 mg/100 g. The average spiked recovery at 3 concentration levels was 98.94%, with a relative standard deviation of 1.58%. The 10 batches of samples were determined by the optimized spectrophotometry and the high performance liquid chromatography reference method. The Bland-Altman consistency analysis indicated that the detection results of the 2 methods exhibited consistent trends.
The method is simple, sensitive, robust against matrix interference and accurate. It is suitable for the rapid determination and quality control of low-content total anthraquinones in health wine with complex matrix.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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