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Determination of oleuropein content in Oleaeuropaea L. related products by high performance liquid chromatography
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Yan-Yu LI1, 2, Ya-Jun WU3, Le-Qi XU1, 2, Min ZHAO4, Hong-Xia CAO4, Ning-Li WANG2, *, Dong PEI1, 2, *
Journal of Food Safety & Quality | 2026, 17(18) : 87 - 95
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Journal of Food Safety & Quality | 2026, 17(18): 87-95
• Special Topic: Functional Foods and Functional Components •
Determination of oleuropein content in Oleaeuropaea L. related products by high performance liquid chromatography
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Yan-Yu LI1, 2, Ya-Jun WU3, Le-Qi XU1, 2, Min ZHAO4, Hong-Xia CAO4, Ning-Li WANG2, *, Dong PEI1, 2, *
Affiliations
  • 1.College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China
  • 2.Research Center for Natural Medicine and Chemical Metrology, Lanzhou Institute of Chemical Physics, ChineseAcademy of Sciences/Key Laboratory for Natural Medicine of Gansu Province, Lanzhou 730000, China
  • 3.Chinese Academy of Quality and Inspection & Testing,Beijing 100176, China
  • 4.Longnan Xiangyu Olive Development Co., Ltd., Longnan 742500, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20260430006
Outline
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Objective

To establish a mothod for determination of oleuropein content in Oleaeuropaea L. related products by high performance liquid chromatography.

Methods

Ultrasonic-assisted extraction was employed to extract oleuropein from samples. The extraction conditions and mobile phase composition were optimized through single-factor experiments. The developed method was subjected to systematic method validation and subsequently applied to the determination of oleuropein content in commercially available products.

Results

The optimal extraction conditions were 30% methanol with a solid-to-liquid ratio of 1:40 (g:mL), extraction temperature of 45 ℃ and extraction for 2 times. The chromatographic conditions consisted of acetonitrile-water (25:75, V:V) with a detection wavelength of 232 nm. Methodological validation demonstrated a good linear relationship (r2=0.999), and the precision [relative standard deviations (RSDs)<4%], repeatability (RSDs<3%) and spiked recovery (87.49%–115.00%) all met the required criteria.The oleuropein content in commercially available products typically ranges from 0 to 10 mg/g.

Conclusion

The method developed is highly sensitive and specific, can overcome complex matrix interferences, and is suitable for determining the content of oleuropein in Oleaeuropaea L. related products.

oleuropein  /  high performance liquid chromatography  /  Oleaeuropaea L. related products
李彦语, 吴亚君, 徐乐琪, 赵敏, 曹红霞, 王宁丽, 裴栋. 高效液相色谱法测定油橄榄相关产品中橄榄苦苷含量. 食品安全质量检测学报, 2026 , 17 (18) : 87 -95 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20260430006
Yan-Yu LI, Ya-Jun WU, Le-Qi XU, Min ZHAO, Hong-Xia CAO, Ning-Li WANG, Dong PEI. Determination of oleuropein content in Oleaeuropaea L. related products by high performance liquid chromatography[J]. Journal of Food Safety & Quality, 2026 , 17 (18) : 87 -95 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20260430006
Appendix
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Year 2026 volume 17 Issue 18
9
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20260430006
  • Receive Date：2026-04-30
  • Online Date：2026-11-05
Article Data
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History
  • Received：2026-04-30
Funding
Affiliations
    1.College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China
    2.Research Center for Natural Medicine and Chemical Metrology, Lanzhou Institute of Chemical Physics, ChineseAcademy of Sciences/Key Laboratory for Natural Medicine of Gansu Province, Lanzhou 730000, China
    3.Chinese Academy of Quality and Inspection & Testing,Beijing 100176, China
    4.Longnan Xiangyu Olive Development Co., Ltd., Longnan 742500, China
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https://castjournals.cast.org.cn/joweb/spaq/EN/10.19812/j.cnki.jfsq11-5956/ts.20260430006
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
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Genus		 种数
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species		 占总种数比例
Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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