Full
Outline
To investigate the hepatotoxic effects of nano-sized and food-grade TiO2 on mice liver.
A total of 80 Kunming mice was randomly divided into the control group and TiO2 exposure groups with different particle sizes. The mice were intragastrically administered with nano-sized titanium dioxide (Nano-TiO2) or food-grade TiO2 (E171) suspension at doses of 50, 150 and 500 mg/kg BW for 14 consecutive days. Purified water was used as the negative control. At the end of the experiment related biochemical indexes were determined, the liver coefficient was calculated and liver antioxidant indexes were detected. Mice AML12 cells were treated with Nano-TiO2 or E171 suspension at concentrations of 0, 25, 50 and 100 μg/mL, and cell viability and intracellular reactive oxygen species (ROS) content were subsequently measured.
After 14 days of exposure, neither nano-sized nor food-grade TiO2 exerted significant effects on body weight, liver coefficient and liver-related biochemical indexes in mice. Nano-TiO2 induced obvious oxidative damage in the liver of mice, while E171 had no significant influence on hepatic antioxidant indexes. In all dose groups, Nano-TiO2 reduced cell viability and increased intracellular ROS levels.Decreased cell viability and elevated ROS content were only observed in the 100 μg/mL E171 treatment group.
Under the research same exposure conditions, the hepatic oxidative toxicity of Nano-sized TiO2 is stronger than that of the food additive E171. This research provides a scientific basis for the safe application of food additive E171.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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