Full
Outline
To prepare Vaccinium spp. anthocyanin microcapsules using gum arabic and maltodextrin as composite wall materials, optimize the encapsulation process, and evaluate the inhibitory activity of crude anthocyanin extract and the microencapsulated product against cancer cells.
Using encapsulation efficiency as the evaluation index, single-factor experiments combined with Box-Behnken response surface methodology were employed to optimize the microencapsulation process. The half-maximal inhibitory concentration (IC50) determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to assess the anti-proliferative effects of the crude anthocyanin extract and the microcapsules on 4 kinds of cancer cell lines.
The optimal process parameters were: maltodextrin proportion 52%, core-wall ratio was 1:7.6 and wall material concentration 13%, yielding an encapsulation efficiency of 97.31%. In vitro activity assays showed that the crude anthocyanin extract significantly inhibited human hepatocellular carcinoma cells (HepG2) and human cervical cancer cells (HeLa) with IC50 of 11.0 μg/mL and 34.4 μg/mL, respectively, exhibiting potent inhibitory effects while showing low toxicity to normal cells. Under the same anthocyanin content, the inhibitory activity of the microcapsules against HeLa and human breast cancer cells (MCF-7) (IC50=9.7 μg/mL and 10.5 μg/mL, respectively) was significantly (P<0.05) better than that of the crude extract (IC50=15.5 μg/mL and 35.1 μg/mL, respectively), and the total product after microencapsulation showed reduced overall toxicity.
The optimized microencapsulation process effectively protects anthocyanins, and the microcapsules exhibit superior inhibitory effects on HeLa and MCF-7 cells compared with the crude extract, providing a new strategy for improving anthocyanin stability and targeted delivery. However, no in vivo experiments are conducted in this study, and further validation is required for its practical application.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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