Full
Outline
Cholera is a severe intestinal infectious disease caused by Vibrio cholerae. Rapid and accurate identification of the pathogen is critical for early warning of outbreaks and emergency response. This review systematically examined recent advances in nucleic acid detection technologies for Vibrio cholerae, focused on the detection principles, technical characteristics and current applications of polymerase chain reaction (PCR) and isothermal amplification techniques, constructed a comprehensive performance comparison table to enable multi-dimensional comparative evaluation; thoroughly analyzed key common challenges in the field, including detection of viable but non-culturable Vibrio cholerae, false-positive interference from dead bacterial DNA, complex matrix inhibition and summarized existing solutions. Based on practical requirements in clinical diagnosis, on-site emergency testing and laboratory-based precision detection, this review proposed scenario-specific technical recommendations:real-time fluorescent quantitative PCR serves as the gold standard for clinical and laboratory testing; loop-mediated isothermal amplification and recombinase polymerase amplification were suitable for rapid on-site detection; and droplet digital PCR was applicable to precise quantification and confirmation of low-load samples. Looking ahead, Vibrio cholerae nucleic acid detection will evolve toward an integrated platform combining propidium monoazide-based viable bacteria discrimination, isothermal amplification, microfluidic integration, multi-target combined detection and intelligent interpretation, thereby enabling faster, more accurate and portable testing that fully meets the practical needs of public health prevention and control.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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