Full
Outline
To establish an extraction process of proteins in Trichiurus lepturus processing by-products by ultrasonic pretreatment-enzyme method and investigate the fundamental properties of the proteins.
Taking Trichiurus lepturus processing by-products as raw materials, the enzymatic extraction conditions of protein by trypsin were optimized through single-factor experiments and orthogonal tests. On this basis, 4 kinds of pretreatment methods including acid pretreatment, alkali pretreatment, thermal pretreatment and ultrasonic pretreatment were applied to Trichiurus lepturus processing by-products, and the process parameters of ultrasonic pretreatment were further optimized via single-factor experiments and orthogonal tests.
Trypsin was the optimum protease, and the optimum enzymolysis conditions were as follows: Enzymolysis temperature 55 ℃,solid-liquid ratio 1:2 (g:mL), enzymolysis time 1.5 h, enzyme dosage 0.5%, the protein recovery of enzymatic hydrolysate was 76.05%±0.69%. The optimum technological conditions of ultrasonic pretreatment were as follows:Ultrasonic power 180 W, ultrasonic time 40 min, ultrasonic temperature 40 ℃. Proteins were extracted under optimal conditions of ultrasound pretreatment with a protein recovery rate of 85.93%±0.62%. The highest content among amino acids was glycine, followed by glutamate and alanine, and had strong 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate) diammonium salt (ABTS) cationic radical, 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activity, and Fe3+ reducing activity.
Ultrasonic pretreatment-enzyme method achieves efficient recovery of protein from Trichiurus lepturus processing by-products. The extracted proteins exhibit strong antioxidant capacity, providing technical support for the high-value utilization of Trichiurus lepturus processing by-products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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