Full
Outline
To establish a method for determining the purine content in prepackaged solid beverages by high performance liquid chromatography and to apply it to the analysis of commercially available ones.
The 4 kinds of purines were extracted from solid beverages using acid hydrolysis. Chromatographic columns were screened based on resolution and peak shape. Detection conditions, including mobile phase pH, flow rate and column temperature, were optimized, and method validation was performed.
Using a Sunniest C18 column, a mobile phase of 10 mmol/L ammonium formate solution at pH 3.68 methanol (99:1, V:V), a flow rate of 1.0 mL/min, a column temperature of 30 ℃, an injection volume of 10 μL, and a detection wavelength of 254 nm, the 4 kinds of purines were completely separated with good peak shapes. Guanine, adenine and hypoxanthine exhibited good linearity in the range of 1–40 mg/L (r2≥0.9990), while xanthine showed good linearity in the range of 0.1–4.0 mg/L(r2=0.9998); the limits of detection were 0.01–0.14 mg/L, and the limits of quantitation were 0.04–0.46 mg/L;spiked recovery rates were 88.00%–113.00%, with relative standard deviations were 0.05%–5.90%. The purine content in the 11 kinds of milk tea samples ranged from 6.66 mg/100 g to 30.46 mg/100 g, and in the 11 kinds of coffee samples, it ranged from 5.28 mg/100 g to 26.11 mg/100 g.
This method exhibits good precision and accuracy and can be used for the simultaneous determination of 4 kinds of purines in solid beverages, providing a scientific basis for dietary choices among individuals with hyperuricemia and gout.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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