Full
Outline
To investigate the effects of adding bacteriophage in controlling the contamination of Lactiplantibacillus plantarum during fermented beer, and optimize the prevention and control effect.
A bacteriophage targeting Lactiplantibacillus plantarum, named LBP-LH, was isolated from rotten apple and duck feces samples, and its characteristics were characterized. By simulating bacterial contamination in fermented beer, Lactiplantibacillus plantarum and Saccharomyces cerevisiae were co-cultured in yeast extract peptone dextrose broth. Then, bacteriophage LBP-LH was added to the co-culture system, and the effects of bacteriophage on preventing Lactiplantibacillus plantarum contamination was evaluated by measuring the ethanol concentration and cell numbers of Saccharomyces cerevisiae and Lactiplantibacillus plantarum in the co-culture system.
Compared with the polluted group, the number of Lactiplantibacillus plantarum in the bacteriophage treatment group decreased significantly from 9 h (P<0.01), while the number of Saccharomyces cerevisiae and ethanol production returned to the control group level (P>0.05); the addition of bacteriophages alone had no significant effect on the growth of Saccharomyces cerevisiae and ethanol fermentation (P>0.05).
The bacteriophage LBP-LH has been verified to specifically eliminate Lactiplantibacillus plantarum without affecting the growth of Saccharomyces cerevisiae and ethanol fermentation. It is safe and can be used as a green biological antibacterial agent for precise prevention and control of lactic acid bacteria contamination during fermented beer. It has practical application potential in the food fermentation industry.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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