Full
Outline
To assess the applicability of the MicroFast® Staphylococcus aureus test strips method for microbiological testing of food products.
This study presented a comprehensive validation of the test chart method, followed the performance specifications set out in the International Organization for Standardization ISO 16140—2: 2016. The validation covered its inclusiveness, exclusiveness and consistency with the test results of the current national standards. The results of both methods were statistically analyzed using t-test and Bland-Altman.
All 30 Staphylococcus aureus target strains showed typical purple colony characteristics on MicroFast test slices; 3 of the 30 non-target strains had atypical blue colonies, and no colony growth was observed colony growth on 27 strains the test slices. After correlation analysis, the MicroFast® Staphylococcus aureus test piece method showed a significant positive correlation with the results of the second method of GB 4789.10—2016 Food safety national standard-Food microbiological test-Staphylococcus aureus test, with a correlation coefficient (r) value of more than 0.976. The suitability of the test slices was also evaluated for a variety of food types, and the calculated β-expected tolerance intervals were all within ±0.5 logarithmic range.
MicroFast® Staphylococcus aureus test pieces have good inclusiveness and exclusivity, consistency and equivalence with current national standard methods, and are suitable for the quantitative detection of Staphylococcus aureus in food.
9
2
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
