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Analysis of flavonoid components in honey by ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry and evaluation of its antioxidant activity
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Jia-Liang ZOU1, 2, Rui XING1, 2, Huan-Huan XU1, 2, Ruo-Lan WANG1, 2, Yuan ZHANG1, 2, Peng WANG1, 2, Hong-Ping CHEN1, 2, Lin CHEN1, 2, Fu WANG1, 2, Yuan HU1, 2, *, You-Ping LIU1, 2, *
Journal of Food Safety & Quality | 2026, 17(12) : 181 - 188
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Journal of Food Safety & Quality | 2026, 17(12): 181-188
• Special Topic: Functional Foods and Functional Components •
Analysis of flavonoid components in honey by ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry and evaluation of its antioxidant activity
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Jia-Liang ZOU1, 2, Rui XING1, 2, Huan-Huan XU1, 2, Ruo-Lan WANG1, 2, Yuan ZHANG1, 2, Peng WANG1, 2, Hong-Ping CHEN1, 2, Lin CHEN1, 2, Fu WANG1, 2, Yuan HU1, 2, *, You-Ping LIU1, 2, *
Affiliations
  • 1.School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
  • 2.Chinese Medicine Germplasm Resources Innovation and Effective Uses Key Laboratory of Sichuan Province,Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20260211001
Outline
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Objective

To establish a method for the flavonoid components of honey from different geographical origins by ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF-MS) and conduct an evaluation of antioxidant activity.

Methods

After sample preparation,UPLC-Q-TOF-MS was employed for analysis. Chemometric methods including principal component analysis(PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to screen differential compounds of honey from different geographical origins. Additionally, the differences in their antioxidant activities were evaluated based on the scavenging rates of 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) cation radical and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical.

Results

The 79 kinds of flavonoid compounds were identified by UPLC-Q-TOF-MS. Honeys from different origins were clearly distinguished, and 17 kinds of flavonoids,including quercetin, nobiletin and kaempferol, were screened as characteristic markers for origin differentiation. Hongqi village honey (HQC) exhibited the strongest scavenging rates against ABTS radical cation and DPPH radical.

Conclusion

The combination of UPLC-Q-TOF-MS and chemometrics can effectively differentiate honey from different geographical origins. This study provides a reference for the authentication of honey origin.

honey  /  different origins  /  ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry  /  flavonoids  /  chemometrics  /  antioxidant activity
邹佳良, 邢睿, 徐欢欢, 王若兰, 张媛, 王鹏, 陈鸿平, 陈林, 王福, 胡媛, 刘友平. 超高效液相色谱-四极杆-飞行时间质谱法分析蜂蜜黄酮类成分及其抗氧化活性评价. 食品安全质量检测学报, 2026 , 17 (12) : 181 -188 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20260211001
Jia-Liang ZOU, Rui XING, Huan-Huan XU, Ruo-Lan WANG, Yuan ZHANG, Peng WANG, Hong-Ping CHEN, Lin CHEN, Fu WANG, Yuan HU, You-Ping LIU. Analysis of flavonoid components in honey by ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry and evaluation of its antioxidant activity[J]. Journal of Food Safety & Quality, 2026 , 17 (12) : 181 -188 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20260211001
Appendix
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Year 2026 volume 17 Issue 12
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20260211001
  • Receive Date：2026-02-11
  • Online Date：2026-11-05
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  • Received：2026-02-11
Funding
Affiliations
    1.School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
    2.Chinese Medicine Germplasm Resources Innovation and Effective Uses Key Laboratory of Sichuan Province,Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
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https://castjournals.cast.org.cn/joweb/spaq/EN/10.19812/j.cnki.jfsq11-5956/ts.20260211001
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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