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Outline
To establish a method for the determination of the monosaccharide components and content in polysaccharides from Liubao tea by ultrasonic-assisted enzymatic extraction-ion chromatography.
Optimization of extraction conditions for monosaccharide components and content in Liubao tea polysaccharides by single-factor experiments and orthogonal experiments. The chromatographic conditions for the determination monosaccharides in Liubao tea polysaccharides by ion chromatography were optimized simultaneously, and the established method was evaluated methodologically.
Through analysis of range and variance, the optimal extraction process conditions for monosaccharide components and content in Liubao tea polysaccharides were that mcellulase to mpectinase ratio of 1:5, enzyme addition level of 4.0%, enzymatic hydrolysis pH of 5.0, solid-to-liquid ratio of 1:25 (g:mL), enzymatic hydrolysis-ultrasonic treatment duration of 40 minutes and enzymatic hydrolysis-ultrasonic temperature at 50 ℃; after methodological evaluation, all 7 kinds of monosaccharides exhibited good linear relationships, with correlation coefficients greater than 0.995, the relative standard deviation of precision was less than 3.2%, the limit of quantitation were satisfactory, and the recovery rates ranged from 83.33% to 101.74%. Through the analysis of monosaccharide components in the polysaccharides of Liubao tea showed that the tea polysaccharides in Liubao tea were composed of rhamnose, arabinose, galactose,glucose, D-xylose, D-mannose and galacturonic acid.
The ultrasonic-assisted enzymatic extraction method can significantly shorten the extraction time of sample, and can be utilized for the rapid determination of monosaccharide components and content in the polysaccharides of Liubao tea.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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