Full
Outline
To establish an pre-column derivatization-high performance liquid chromatography(HPLC) method for the determination of D-mannose content.
p-aminobenzoic ethyl ester (p-ABEE) was selected as the derivatization agent. The derivatization reaction conditions between D-mannose and p-ABEE were systematically optimized, and the effects of molar ratio of derivatization reactants, reaction temperature and reaction time on derivatization efficiency were emphatically investigated. Meanwhile, the HPLC detection conditions,including chromatographic column type and mobile phase composition, were optimized to realize efficient separation and accurate quantification of derivatized products.
The optimal experimental conditions were determined as follows: the molar ration of D-mannose to p-ABEE was 1:54, and the reaction was performed at a constant temperature of 75 ℃ for 1.5 h. The mobile phase consisted of 0.02% trifluoroacetic acid (TFA) and acetonitrile(77:23, V:V) with gradient elution at a flow rate of 1.00 mL/min. The detection wavelength was determined to be 307 nm. and the column temperature was maintained at 35 ℃. Method validation results showed that D-mannose had a good linear relationship in the concentration range of 0.50–20.01 mmol/L (r2=0.998) with the limit of detection and limit of quantification were 0.05 mmol/L and 0.12 mmol/L, respectively. The recoveries of D-mannose were ranged from 98.55% to 101.45%, with the relative standard deviations of 3.62%–12.74% (n=3).
This method is simple to operate, with mild derivatization conditions and excellent separation effect. It also exhibits good linearity, high recovery rate and low detection limit, showing strong practicality. The method is suitable for the determination of D-mannose in in various matrices such as polysaccharides extracted from plants.
12
3
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
