Full
Outline
To establish an analytical method for the simultaneous determination of 50 kinds of mycotoxins in Dimocarpus longan Lour. and its dried products by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).
Samples were ultrasonically extracted with formic acid-acetonitrile-water (1:79:20, V:V:V), and purified with an Oasis PRiME HLB solid-phase extraction column.Chromatographic separation was performed on a Waters CORTECS T3 column (2.1 mm×100 mm, 2.7 μm) with gradient elution using 0.1% formic acid aqueous solution and methanol-acetonitrile (4:6, V:V) as mobile phases.Detection was carried out in positive and negative electrospray ionization modes with multiple reaction monitoring, and quantification was achieved using the matrix-matched external standard method.
Under optimized conditions, the 50 kinds of mycotoxins exhibited good linearity in a certain mass concentration (r2>0.995). The limits of detection and limits of quantitation were 0.03–3.48 μg/kg and 0.10–11.59 μg/kg, respectively. The average recoveries at 3 spiked levels (low, medium and high) ranged from 76.3%–119.6%, with relative standard deviations of 0.45%–9.02%. The established method was applied to the analysis of 62 batches of Dimocarpus longan Lour. and its dried products and 16 kinds of mycotoxins were detected in 21 samples, with the average contents ranging from 0.42–13.51 μg/kg.
The established method is simple in operation, high in sensitivity and accurate in results, which is suitable for high-throughput screening and quantitative detection of 50 kinds of mycotoxins in Dimocarpus longan Lour. and its dried products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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