Full
Outline
To establish an analytical method for the simultaneous determination of 12 kinds of organic acids (tartaric acid, hydroxycitric acid, lactic acid, citric acid, succinic acid, fumaric acid, gallic acid, protocatechuic acid, chlorogenic acid, caffeic acid, syringic acid and ferulic acid) in Hibiscus sabdariffa L. wine by solid-phase extraction and high performance liquid chromatography.
Mixed-mode strong anion exchange polymeric anion exchange (PAX) solid-phase extraction cartridges were used for purification and enrichment. The samples were eluted with 0.1% formic acid in water and methanol as mobile phases and separated on a CAPECELL PAK ADME C18 liquid chromatography column (4.6 mm×250 mm, 5 μm), and a diode array multi-wavelength detection were employed.
The 12 kinds of organic acids were effectively separated; there was a good linear relationship within the linear range of various mass concentrations, with correlation coefficients (r) all above 0.9998;the average spiked recovery rates ranged from 82.78%–101.52%, with relative standard deviations of 0.90%–4.79%. The limits of detection were 0.00047–0.49392 mg/L and the limits of quantitation were 0.00157–1.64640 mg/L.
This method is simple to operate, good separation effect, accurate data and high sensitivity, making it suitable for the quantitative and qualitative detection of organic acids in Hibiscus sabdariffa L. wine.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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