Full
Outline
To develop a method for the rapid quantitative detection of ochratoxin A in grain, oil crops and nuts by quantum dot fluorescence.
Using polystyrene microsphere quantum dot loading technology, a quantum dot microsphere probe with high fluorescence intensity was constructed by covalent coupling, ochratoxin A monoclonal antibody was coupled to the probe surface by activation coupling method, and the quantum detection system of ochratoxin A was constructed.
Through the optimization of experimental parameters, the established quantum dot fluorescence detection system showed excellent analytical performance for ochratoxin A.The linear relationship was good in the range of 1–20 ng/mL (r2>0.99), and the limits of detection and limits of quantitation were as low as 0.5 ng/mL and 2.0 ng/mL. The method had strong specificity, there was not significant cross reaction with common mycotoxins such as deoxynivalenol and zearalenone. The method was validated by methodology according to the standard of LS/T 6142—2023 Evaluation protocol for rapid detection method of mycotoxins in grains, spiked recovery rates were 90.3%–114.5%, and the relative standard deviation was less than 15%, the method of accuracy and precision met the requirements of the detection specification about ochratoxin A.
The quantum dot fluorescence detection system for ochratoxin A has the advantages of high sensitivity, good specificity, simple operation and rapid analysis, it is suitable for rapid and accurate quantitative detection of ochratoxin A in grain, oil crops and nuts, it provides an effective technical means for the field screening and monitoring of mycotoxins in agricultural products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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