Full
Outline
To establish a method for the detection of gamithromycin residues in Oryctolagus cuniculus meat by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).
Oryctolagus cuniculus meat samples were extracted using acetonitrile, subjected to defatting with n-hexane, and further purified with Qasis MCX solid-phase extraction cartridges, and analyzed by Xevo TQ-XS UPLC-MS/MS with gradient elution, electrospray ionization positive scan mode (ESI+), multiple reaction monitoring(MRM) and internal standard method were applied for quantitative analysis.
There was an excellent linear correlation between the target peak area and the injection mass concentrations of gamithromycin at the range of 0.25–50.00 ng/mL (r2>0.999). The limit of detection and limit of quantitation were 0.25 μg/kg and 0.50 μg/kg. The average recoveries ranged from 76.8% to 102.3%, with intra-batch and inter-batch relative standard deviations both lower than 15% (n=6) at the addition levels of 0.5, 1.0 and 50.0 μg/kg.
The method is sensitive, accurate, and suitable for the detection of residues of gamithromycin in Oryctolagus cuniculus meat. This study can provide scientific basis and technical support for the monitoring and quality safety control of veterinary drug residues in Oryctolagus cuniculus meat products.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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