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Detection of oxytetracycline residues in food by carbon dots fluorescent probes
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Hui WANG1, *, Cheng-Qian LI1, 2, Xiao-Yu LUO1, 2, Yong HAN1, *
Journal of Food Safety & Quality | 2026, 17(9) : 225 - 232
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Journal of Food Safety & Quality | 2026, 17(9): 225-232
• Special Topic: Analysis and Monitoring of Toxic and Harmful Substances in Food •
Detection of oxytetracycline residues in food by carbon dots fluorescent probes
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Hui WANG1, *, Cheng-Qian LI1, 2, Xiao-Yu LUO1, 2, Yong HAN1, *
Affiliations
  • 1.School of Agriculture and Bioengineering, Longdong University, Qingyang 745000, China
  • 2.College of Life Sciences and Engineering, Lanzhou University of Technology, Lanzhou 730050, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251229007
Outline
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Objective

To establish a method for rapid detection oxytetracycline (OTC) residues in animal-derived foods by carbon dots (CDs) fluorescent probe.

Methods

Blue-emissive carbon dots (B-CDs) were synthesized via a one-step hydrothermal method using salicylic acid and o-phenylenediamine as precursors, and a fluorescent probe for OTC detection was constructed.

Results

The as-prepared B-CDs exhibited near-spherical morphology, uniform size distribution, good water dispersibility and stable fluorescence. The fluorescence of B-CDs could be selectively quenched by OTC. Under optimized conditions, the fluorescent probe exhibited good linear response and sensitivity for OTC in the range of 0–1.00 mg/kg (Y=1070.95–673.35X, r2=0.9959), with a limit of detection as low as 3.60 μg/kg and a limit of quantitation of 11.99 μg/kg. In spiked recovery tests using real samples, recoveries ranged from 94.00% to 102.00%, with relative standard deviations between 0.17% and 2.25%.

Conclusion

The proposed method is simple to operate, highly sensitive, reliable and cost-effective, demonstrating promising potential for rapid screening of OTC residues in food samples.

carbon dots  /  fluorescent probe  /  rapid detection  /  oxytetracycline
汪慧, 李承谦, 罗晓羽, 韩雍. 碳点荧光探针检测食品中的土霉素残留. 食品安全质量检测学报, 2026 , 17 (9) : 225 -232 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251229007
Hui WANG, Cheng-Qian LI, Xiao-Yu LUO, Yong HAN. Detection of oxytetracycline residues in food by carbon dots fluorescent probes[J]. Journal of Food Safety & Quality, 2026 , 17 (9) : 225 -232 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251229007
Appendix
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Year 2026 volume 17 Issue 9
11
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251229007
  • Receive Date：2025-12-29
  • Online Date：2026-11-05
Article Data
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History
  • Received：2025-12-29
Funding
Affiliations
    1.School of Agriculture and Bioengineering, Longdong University, Qingyang 745000, China
    2.College of Life Sciences and Engineering, Lanzhou University of Technology, Lanzhou 730050, China
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表12种不同金属材料的力学参数
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Family		 属数
Number of
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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