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Optimization of enzymatic hydrolysis conditions for determining tryptophan in special medical purpose formula milk powder
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Wen-Hui， HU1, 2, Shuo TANG1, 2, *, Hong YUE1, 2, Xiao-Yan HUANG1, 2, Jian-Ying LIANG1, 2, Li-Jun LIU1, 2, Xiao-Li WU1, 2
Journal of Food Safety & Quality | 2026, 17(9) : 191 - 198
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Journal of Food Safety & Quality | 2026, 17(9): 191-198
• Special Topic: Functional Foods and Functional Components •
Optimization of enzymatic hydrolysis conditions for determining tryptophan in special medical purpose formula milk powder
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Wen-Hui， HU1, 2, Shuo TANG1, 2, *, Hong YUE1, 2, Xiao-Yan HUANG1, 2, Jian-Ying LIANG1, 2, Li-Jun LIU1, 2, Xiao-Li WU1, 2
Affiliations
  • 1.Inner Mongolia Yili Industrial Group Co., Ltd., Hohhot 010110, China
  • 2.Key Laboratory of Bovine and Ovine Milk and Meat Product Risk Prevention and Key Technology, State Administration for Market Regulation, Hohhot 010110, China
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251224005
Outline
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Objective

To establish a method for determining tryptophan in special medical purpose formula milk powder by enzymatic hydrolysis based on amino acid analyzer.

Methods

Based on single-factor experiments, the Box-Behnken response surface methodology was employed, with enzyme addition amount, enzymatic hydrolysis temperature and enzymatic hydrolysis time as influencing factors, and tryptophan recovery rate as the response value. The optimal enzymatic hydrolysis conditions were determined, and the effects of different purification methods on the results were also compared.

Results

The optimal enzymatic hydrolysis conditions were: Enzyme addition amount 0.74 mL, enzymatic hydrolysis temperature 45.2 ℃ and enzymatic hydrolysis time 12 h. Through experimental comparison, 12% trichloroacetic acid was identified as the optimal precipitant. Under the optimized conditions: Methodological validation showed a tryptophan recovery rate of 95.5%–101.0%, with relative standard deviations (RSDs, n=7) ranging from 0.9% to 1.6%, and limit of detection (LOD) andlimit of quantitation (LOQ) of 0.3 mg/100 g and 1.0 mg/100 g, respectively.

Conclusion

This method offers ease of operation, high accuracy and excellent precision, making it suitable for the quantitative determination of tryptophan in special medical purpose formula milk powder.

tryptophan  /  amino acid analyzer  /  enzymatic hydrolysis  /  special medical purpose formula milk powder  /  response surface methodology
胡文慧, 唐烁, 岳虹, 黄晓燕, 梁建英, 刘丽君, 吴晓莉. 特殊医学用途配方乳粉中色氨酸测定酶解条件优化. 食品安全质量检测学报, 2026 , 17 (9) : 191 -198 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251224005
Wen-Hui， HU, Shuo TANG, Hong YUE, Xiao-Yan HUANG, Jian-Ying LIANG, Li-Jun LIU, Xiao-Li WU. Optimization of enzymatic hydrolysis conditions for determining tryptophan in special medical purpose formula milk powder[J]. Journal of Food Safety & Quality, 2026 , 17 (9) : 191 -198 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20251224005
Appendix
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Year 2026 volume 17 Issue 9
9
2
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20251224005
  • Receive Date：2025-12-24
  • Online Date：2026-11-05
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History
  • Received：2025-12-24
Funding
Affiliations
    1.Inner Mongolia Yili Industrial Group Co., Ltd., Hohhot 010110, China
    2.Key Laboratory of Bovine and Ovine Milk and Meat Product Risk Prevention and Key Technology, State Administration for Market Regulation, Hohhot 010110, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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