Full
Outline
To separate and identify the contaminated yeast that causes the fruit yogurt to bulge and establish a rapid detection method for the main contaminated yeast.
Yeasts were isolated from different samples of fermented yogurt and theirs gas production characteristics were also analyzed. Molecular biology methods were used to identify isolated strains. Specific primers were designed based on the 26S rDNA sequence, and real-time fluorescence quantitative polymerase chain reaction using SYBR Green I dye was applied for the rapid detection yeasts.
The 9 yeast strains were isolated from 3 samples of Vaccinium uliginosum L. yogurt, Mangifera indica L. fruit yogurt and Prunus persica ‘Yellow Peaches’ fruit yogurt. The 9 yeasts were identified as Wickerhammomyces anomalus, Pichia inconspicua, Clavispora lusitaniae and Pichia cactus through molecular biology methods. Among them, Clavispora lusitaniae was the main dominant yeast and had the strongest gas production ability. The primer designed based on the 26S rDNA gene had good specificity. The correlation coefficients of the constructed standard curves were all greater than 0.99. The sensitivity of this method for detecting yeast was 102 CFU/mL. The coefficient of variation within and between groups in the repeatability test was less than 2%, indicating that the method had good reliability and stability.
In this study, the main contaminating yeast causing gas production in fruit milk is Clavispora lusitaniae. The developed detection method can be used for rapid detection of yeast in fruit fermented milk. It provides a technical support for the food safety of fermented fruit milk.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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