Full
Outline
To optimize the detection method for vitamin B1 (VB1) in infant formula milk powder.
The content of VB1 in different matrix samples was determined by ultra performance liquid chromatography. The effects of hydrolysis cooling time, hydrolysis temperature, production process, enzyme solution and raw material matrix on the detection results were analyzed to optimize the pretreatment for VB1 detection in infant formula milk powder.
The detection process was accurate and reliable, with recovery rates of 94.3% to 102.0% for VB1 standard solutions without milk powder matrix. Hydrolysis cooling time, production process and mixed enzyme solution had no significant effect on VB1 detection results and recovery rate (P>0.05). Hydrolysis temperature and milk powder matrix were key synergistic factors causing lower detection results. VB1 detection results and recovery rate from room-temperature hydrolysis were significantly higher than those from high-temperature hydrolysis (P<0.01). Furthermore, recovery rate of mixed powders decreased significantly after high-temperature hydrolysis (P<0.05).
The optimized ambient-temperature hydrolysis method eliminates high-pressure hydrolysis operations, simplifies pretreatment procedures and achieves spiked recovery rates approaching 100%. This method is suitable for the efficient batch detection of VB1 in infant formula milk powder.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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